Low-intensity laser radiation increased the content of anthocyanin, kaempferol, quercetin, and anthocyanin in leaves of Arabidopsis thaliana plants at 5 weeks of age ( Dudareva et al., 2020). In plant adaptation to the external environment, phenolics can eliminate free radicals, avoid membrane peroxidation, reduce plant damage caused by external stress, etc., which has an irreplaceable role for the entire ecosystem. Studying the effects of enhanced UV-B radiation on organisms and formulating effective protective measures has become a significant issue in the process of crop cultivation in the world ( Cloix et al., 2012). Plants’ biological responses to UV-B were influenced by spectral UV-B, exposure continuity, ascendancy conditions, and species-specific genotypes ( Ibañez et al, 2017 Lingwan and Masakapalli, 2022). Many studies have found that UV-B exposure can trigger gene activity and regulatory responses at low levels ( Hara et al., 2019). As a result of excessive UV-B radiation, plant growth and development can be negatively affected, and their physiological metabolism can be adversely affected. UltraViolet- Biological radiation reaching the earth’s surface increased as the atmospheric ozone layer is destroyed (280 to 320 nm). However, the higher dose of UV-B radiation (T2) will cause greater damage to the pigmented potatoes plants, making the plants reduce the yield, and significantly reduce the tuber nutrients.ĭiscussion: This study showed that proper ultraviolet radiation will not harm pigmented potatoes, but also improve their oxidative stress tolerance, increase the structure genes expression of anthocyanins and continuously synthesize beneficial substances to improve the yield and quality of potato tubers. The number of nutrients and gene expression in tubers raised by the “Huaxinyangyu” was the highest at 84 days, and “Jianchuanhong” was the highest at 72 days. phenols, chlorogenic acids, flavonoids, vitamin C, anthocyanins) and increased the expression of structural genes for anthocyanin synthesis. In addition, low-dose UV-B treatment promoted the synthesis of tuber nutrients (e.g. plant height, pitch, stem diameter, and root shoot ratio) of plants treated with low dose ultraviolet (T1) did not change significantly compared with the absence of ultraviolet radiation (CK), even unit yield increased slightly Similarly, under low UV-B radiation, photosynthetic and physiological parameters (photosynthetic rate, stomatal conductance, respiration rate, and transpiration rate) of leaves were significantly increased. Results: Pearson correlation analysis and principal component analysis showed that the agronomic characters (i.e. Methods: To investigate the phenotypic changes, physiological responses, and internal genes expression of potatoes under enhanced UV-B radiation, two Yunnan native pigmented potatoes varieties named “Huaxinyangyu” and “Jianchuanhong” were exposed to different UV-B doses during whole growth duration. Introduction: UltraViolet- Biological (UV-B) plays an important role in plant growth and the formation of nutrients, especially secondary metabolites. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, Yunnan, China.As a result, your scientists can switch entirely to SnapGene without losing data, or can continue using legacy software together with SnapGene without conflict.Īs a service to the research community, SnapGene provides tutorial videos along with a library of carefully annotated plasmids, along with guides to popular cloning methods.Xiaojie Wu, Bicong Chen, Jiping Xiao * and Huachun Guo SnapGene supports a host of file formats.SnapGene automatically generates a record of every sequence edit and cloning procedure, so you won’t lose track of how a construct was made, even after a lab member leaves.dna files can be opened by the free cross-platform SnapGene Viewer, enabling you to share richly annotated maps and sequences with colleagues. Every DNA manipulation in SnapGene is automatically recorded, so you can see exactly what you did and retrieve the sequences of ancestral constructs.SnapGene makes your DNA manipulations easy to visualize and simulate, and alerts you to errors before they happen.The software also enables documentation and sharing of data. With an intuitive interface, the software enables DNA sequence visualization, sequence annotation, sequence editing, cloning, protein visualization, and simulating common cloning methods. SnapGene enables an easy and secure way to plan, visualize, and document everyday molecular biology procedures.
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